Expression and function of the luteinizing hormone choriogonadotropin receptor in human endometrial stromal cells

Expression and function of the luteinizing hormone choriogonadotropin receptor in human endometrial stromal cells

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Abstract The human luteinising hormone choriogonadotropin receptor (LHCGR) is a G-protein coupled receptor activated by both human chorionic gonadotropin (hCG) and luteinizing hormone (LH), ECONOMIC REALITIES AND PROSPECTS OF BRICS AND G7 two structurally related gonadotropins with essential roles in ovulation and maintenance of the corpus luteum.LHCGR expression predominates in ovarian tissues where it elicits functional responses through cyclic adenosine mononucleotide (cAMP), Ca2+ and extracellular signal-regulated kinase (ERK) signalling.LHCGR expression has also been localized to the human endometrium, with purported roles in decidualization and implantation.However, these observations are contentious.

In this investigation, transcripts encoding LHCGR were undetectable in bulk RNA sequencing datasets from whole cycling endometrial tissue and cultured human endometrial stromal cells (EnSC).However, analysis of single-cell RNA sequencing data revealed cell-to-cell transcriptional heterogeneity, and we identified a small subpopulation of stromal cells with detectable LHCGR transcripts.In HEK-293 cells expressing recombinant LHCGR, both hCG and LH elicited robust cAMP, Ca2+ and ERK signals that were absent in wild-type HEK-293 cells.However, none of these responses were recapitulated in primary EnSC cultures.

In addition, proliferation, viability and decidual transformation Quality by Design Approach for Development and Characterization of Granisetron Hydrochloride-Loaded Orodispersible Films of EnSC were refractory to both hCG and LH, irrespective of treatment to induce differentiation.Although we challenge the assertion that LHCGR is expressed at a functionally active level in the human endometrium, the discovery of a discrete subpopulation of EnSC that express LHCGR transcripts may plausibly account for the conflicting evidence in the literature.